Abstract
Purpose
The high mobility group proteins (HMGs) include the HMGI family members HMGI-C and HMGI(Y) that their expression in adult tissues generally correlates with malignant tumor phenotypes. The aim of this study was to assess the relationship of HMGI-C or HMGI(Y) gene expression and prognosis in colorectal cancer patients.
Method
The gene expressions of HMGI-C and HMGI(Y) in 31 paired samples of colorectal tumor and corresponding non-tumor were determined by real-time RT-PCR.
Result
The expression of HMGI(Y) in tumor of colorectal cancer was associated with Duke¡¦s staging (p=0.044), while, in non-tumor, the expression of this gene was significant with metastasis (p=0.003). Patients with Duke¡¦s stage A&B present high HMGI(Y) expression in non-tumor of colorectal cancer (p=0.006). However, in Duke¡¦s stage C&D, patients present high HMGI-C expression in colorectal tumor (p=0.023). In non-metastasis group, HMGI(Y) was highly expressed in non-tumor of colorectal cancer. However, in metastasis group, there was no significant difference between tumor and non-tumor tissues in both HMGI-C and HMGI(Y) gene expressions.
Conclusion
The HMGI-C and HMGI(Y) quantitations by real-time RT-PCR are associated with Duke's staging and metastasis; hence, the gene expression levels may useful in clinical practice.
