Inflammation gene polymorphisms contributing to pro-atherosclerosis and pro-inflammation processes of infracted hearts were intensively investigated. In this study, we performed a case-control study involving 117 acute myocardial infarction (AMI) patients versus 165 unrelated normal subjects to distinguish associations of the onset of AMI with 252A/G, 804C/A polymorphisms of Lymphotoxin-alpha(LTA, also named TNF-?) gene, and 3279C/T polymorphism of its binding partner, LGALS2 gene. An association concordant to the original study between LTA 252G and 804A homozygosity with the onset of AMI was found. In addition, 804C/A polymorphism more generally correlated to the prevalence of coronary artery disease (CAD) rather than the onset of AMI. The susceptible haplotype of LTA to AMI risk was found as the 252G allele couple with 804C or 804A allele among the Taiwanese population. More population- based studies are warranted to confirm these findings and evaluate its clinical impact on AMI diagnosis and prognosis.